TABLE 1.
E. coli strains, plasmids, and phages used in this study
| Strain, plasmid, or phage | Relevant genotype or phenotype | Reference or source |
|---|---|---|
| E. coli strains | ||
| JM83 | ara Δ(lac-proAB) rpsL φ80dlacZΔM15 | 33 |
| JM109 | recA1 gyrA96 thi hsdR17 relA1 supE44 Δ(lac-proAB)/F′ traD36 proAB lacIqZΔM15 | 33 |
| CJ236 | dut-1 ung-1 thi-1 relA1/pCJ105 (Cmr) | 17 |
| BMH71-18 | Δ(lac-proAB) thi supE mutS215::Tn10 (Tcr)/F′ traD36 proAB lacIqZΔM15 | 17 |
| NF83 | recA56 ara Δ(lac-proAB) rpsL φ80 dlacZΔM15 | 7 |
| TN102 | W3110 Nalr | 12 |
| BL21(DE3) | ompT hsdS gal/λ DE3 lacUV5-T7 gene 1 | 29 |
| Plasmids | ||
| pUC119 | Apr, lacZ′ | 33 |
| pET11a | Apr, T7 promoter | 29 |
| pKK641A′ | Kmr, R64drd-11 derivative carrying 18.5-kb BglII-HindIII pil segment and rep sequence | 12 |
| pKK641A′ pilS1 | pKK641A′ carrying the pilS1 mutation | This work |
| pKK661 | Cmr, pHSG576 derivative carrying 35.6-kb HindIII-BglII tra segment of R64drd-11 | 12 |
| pKK692 | 0.8-kb HincII-BglII pilS+ fragment in pUC119 | 37 |
| Phages | ||
| Iα | 4 | |
| PR64FS | 5 |