Table 1.
Detection of cleaved Stx2a in STEC infected patients’ sera.
| Pt * | Sex | Age (y) |
Clinical Symptoms |
E. coli Serogroup |
Toxin Type |
RT-PCR Stx2 Gene |
Stx2 by ELISA (ng/mL) |
Cleaved Stx2 by LCFTS (ng/mL) |
Neutrophil- Bound Stx2 |
|---|---|---|---|---|---|---|---|---|---|
| 1 | f | 1.7 | BD, D, H, P, HUS | O26 | Stx2 | + | 3.36 | 2.90 | + |
| 2 | f | 5.6 | BD, D, P, HUS | O157 | Stx2 | + | 3.37 | 11.45 | + |
| 3 | m | 8.3 | BD, H, P, HUS | O127 | Stx2 | + | 2.41 | - | + |
| 4 | f | 10.2 | BD, D, P | O145 | Stx2 | − | 2.23 | 1.98 | + |
| 5 | m | 10.2 | BD | O8 | Stx2 | + | 2.38 | - | − |
| 6 | m | 3.3 | BD, D, P | nd | Stx2 | − | 2.13 | - | − |
| 7 | m | 6.4 | H, P | O127 | Stx2 | + | 3.00 | 8.27 | + |
| 8 | m | 5.7 | D, H, P | O127 | Stx2 | + | 3.02 | - | + |
* Clinical and microbiological features of 8 STEC-infected patients: sex (3 females and 5 males); age (median 5.7 years, range 1.7–10.2 years); clinical symptoms as bloody diarrhea (BD, 6/8) and/or diarrhea (D, 5/8), hematuria (H, 4/8), proteinuria (P, 7/8), hemolytic uremic syndrome (HUS, 3/8); E. coli serogroup determination; toxin type according to the presence of the gene encoding Stx2 in fecal extracts identified by real time-polymerase chain reaction (RT-PCR) or by direct identification of the toxin type in serum by enzyme-linked immunosorbent assay (ELISA, ng/mL); cleaved Stx2 identified by luminometric cell-free translation system (LCFTS, ng/mL); neutrophil-bound Stx2 (6/8).