TABLE 1.
NMR data for the major component in the phospholipid fraction from Corynebacterium sp. strain LMG 3820
| Component | System | 1H shift | Multiplicity (couplings) | 13C shifta |
|---|---|---|---|---|
| Glycerol moieties | CH2Ob | 4.42 | dd (3.5, 12.0) | 60.9 |
| 4.19 | dd (6.6, 12.0) | |||
| CHO | 5.23 | m | 68.5 | |
| CH2O | 3.99 | m | 61.7 | |
| CH2Ob | 4.14 | d (5.8) | 63.1 | |
| CHO | 3.98 | m | 66.9 | |
| CH2Oc | 3.95 | ddd (3.5, 7.9, 11.1) | 65.1 | |
| 3.87 | ddd (5.6, 8.1, 10.7) | 65.1 | ||
| Fatty acids | CH3 | 0.89 | t (7.2) | 13.5 |
| CH2 | 1.27 | m | 22.3 | |
| ..(CH2)x.. | 1.27–1.35 | m | 28.8–29.3 | |
| CH2 | 1.63 | m | 24.6 | |
| CH2 | 2.34 | m (several close together) | 34.8, 34.9 | |
| CO | 173.1, 173.8 | |||
| ..CH2 | 1.31 | m | 28.8–29.3 | |
| CH2 | 2.02 | m | 26.8 | |
| CH⩵CH | 5.35 | m | 129.2 | |
| CH2 | 2.02 | m | 26.8 | |
| CH2.. | 1.31 | m | 28.8–29.3 | |
| ..CH (CH3) | 2.04 (CH) | m | 36.5 | |
| 0.94 (CH3) | d (6.8) | 20.3 | ||
| CH⩵CH | 5.22d | dd (6.3, 15.7) | 135.8 | |
| 5.33 | dt (6.3, 15.7) | 128.1 | ||
| CH2 | 1.96 | m | 31.9 | |
| CH2.. | 1.31 | m | 28.8–29.3 |
a
Taken from the one-bond (HMQC) and long-range (HMBC) 13C-1H correlations.
b
The protons of the methylene show no couplings to 31P.
c
The protons of the methylene group show couplings to 31P, which shows a single resonance at +2.2 ppm relative to external H3PO4.
d
Olefinic carbon adjacent to aliphatic methene carbon-carrying methyl group.