Table 1.
Study Schedule.
| Event | Days Pre-Inoculation | Days Post-Inoculation (PI) | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| −9 | −8 | −7 to −2 | −1 | 0 | 1–2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12–13 (T f) |
|
| Veterinary Release | X | |||||||||||||||
| Move to Containment | X | |||||||||||||||
| Acclimation | X | X | X | X | ||||||||||||
| Telemetry a | X | X | X | X | X | X | X | X | X | X | X | X | X | X | X | X |
| MARV Challenge/Mock Exposure | X | |||||||||||||||
| Tether Assembly | X | |||||||||||||||
| Catheter Connection | X | |||||||||||||||
| Awake Observations | X | X | X | X | X | X | X | X | X | X | X | X | X | X | X | |
| Observation (Anesthetized) | X | X | X | |||||||||||||
| Health Status Checks b | X | X | X | X | X | |||||||||||
| Plaque Assay | X c | X | X | X | X | X | ||||||||||
| Polymerase Chain Reaction (PCR) | X | X c | X d | X d | X | X | X | X | X | |||||||
| Hematology | X c | X | X | X | X | X | X | X | ||||||||
| Clinical Chemistry | X c | X | X | X | X | X | X | X | ||||||||
| Coagulation | X c | X | X | X e | X | X | X | X | ||||||||
a Telemetry baseline data collection started on Day −6 PI. b Health status checks (i.e., additional cage-side observations) began when animals showed clinical signs (responsiveness score ≥ 1) and were discontinued when all surviving animals had a responsiveness score = 0. c Day 0 samples were collected prior to challenge. d Two PCR samples were collected (a.m. and p.m.). e Two animals (animal 4 from the mock-exposed group, and animal 11 from the MARV-exposed group) weighed less than 3.6 kg on Day 0; therefore, coagulation analysis was not performed on those animals on Day 5 PI. f T = terminal (moribund, found deceased, or end-of-study). Note: For 7 MARV-exposed animals, terminal blood collection for plaque assay, PCR, hematology, clinical chemistry, and coagulation occurred on Day 8 PI (6 animals) or Day 10 PI (1 animal). Blood was not collected on animals found deceased.