Table 2.
Kinetic parameters of enzymatic thiosulfinate production.
DMTS | DETS | DPTS | DATS | |
---|---|---|---|---|
Initial turnover rate 1, s−1 | 0.23 ± 0.01 | 1.03 ± 0.09 | 0.97 ± 0.04 | 1.56 ± 0.04 |
Turnover rate at half-conversion, s−1 | 0.07 ± 0.002 | 0.39 ± 0.01 | 0.39 ± 0.01 | 0.75 ± 0.08 |
Half-conversion time, min | 231 | 29 | 29 | 14 |
Reaction slowdown rate 2 | 70% | 62% | 60% | 52% |
1 The concentration of MGL active sites was 1.95 μM. 2 Relative decrease in the turnover rate after reaching a half-conversion time.