Figure 1.

Lysicamine induces cell toxicity and reduces migration in thyroid tumor cells. (a) Molecular structure of lysicamine, synthesized via benzyne chemistry [27]. (b) A reduction in cell viability was observed in KTC-2, HTH83, BCPAP (TC cell lines), and Nthy-ORI (thyroid non-tumoral cell line) 72 h after treatment with three concentrations of lysicamine. Cell viability was measured via PrestoBlue assay and expressed as a percentage of viability compared to non-treated cells. (c) Representative images of colony assay across all TC cell lines 72 h after lysicamine IC₅₀ treatment vs. no treatment control (DMSO) and subsequent 10 days of culture without treatment. (d) Representative images of wound closure progression 12 h after lysicamine treatment of KTC-2 at IC₅₀ concentration. For (b), data were plotted as mean ± SEM of at least three biological replicates in triplicate; significance was determined by two-way ANOVA multiple comparisons followed by Bonferroni test (post-test); ** p < 0.001 *** p < 0.0001.