Figure 2.

Lysicamine induces cell toxicity and reduces thyroid cancer cell spheroids area. (a,b) A reduction in cell viability was observed in spheroids of KTC-2 (a) on day 14 and in HTH83 (b) on days 10 and 14 after 2× IC₅₀ lysicamine treatment. Spheroid viability was measured via PrestoBlue assay and expressed as a percentage compared to untreated control. (c,e) Reduction in KTC-2 (c) and HTH83 (e) spheroids area after 2× IC₅₀ lysicamine treatment. (d,f) Representative images of KTC2 (d) and HTH83 (f) spheroids at days 0, 3, and 10 after treatment with 2× IC₅₀ lysicamine vs. DMSO. The area of the spheroids from the images was quantified by ImageJ, Version 1.53t (imagej.nih.gov) and expressed as a percentage of untreated control. Data were plotted as mean ± SEM of at least three biological replicates in triplicate; significance was determined by two-way ANOVA multiple comparisons followed by Bonferroni test (post-test); * p < 0.05; *** p < 0.0001.