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Purification of B. cepacia HBQ reductase
| Fraction | Vol (ml) | Act (U/ml) | Total activity (U) | Protein (mg/ml) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|---|---|
| Crude extract | 44 | 449.2 | 19,769 | 6.6 | 68 | 100 | 1.0 |
| Butyl-Sepharose | 50 | 52 | 2,600 | 0.7 | 74.3 | 13.1 | 1.1 |
| Blue-Sepharose (concentrated) | 0.8 | 1,750 | 1,400 | 3.76 | 466 | 7.1 | 6.8 |
| Sephacryl S100 (concentrated) | 0.4 | 952.5 | 381 | 0.52 | 1,831 | 1.9 | 26.9 |
