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Purification of B. cepacia HQDO
| Fraction | Vol (ml) | Activity (U/ml) | Total activity (U) | Protein (mg/ml) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|---|---|
| Crude extract | 10 | 3.52 | 35.2 | 8.6 | 0.4 | 1.0 | |
| 35–50% (NH4)2SO4 | 5 | 7.9 | 39.5 | 7.2 | 1.09 | 100 | 4.75 |
| Q-Sepharose (concentrated) | 1 | 18.8 | 18.8 | 9.5 | 1.99 | 53.4 | 4.97 |
| Resource-Phenyl (concentrated) | 0.08 | 180 | 14.4 | 4.9 | 36.7 | 40.9 | 91.7 |
