TABLE 1.
Bacterial strains, cosmids, plasmids, and phage used in this study
| Strain, cosmid, plasmid, or phage | Relevant genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | GIBCOBRL |
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara leu)7697 galU galK λ−rpsL nupG | GIBCOBRL |
| STBL2 | F−mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 thi supE44 relA1 λ− (lac-proAB) | GIBCOBRL |
| M. smegmatis | ||
| mc2155 | ept-1 | 42 |
| mc2848 | ept-1 fxbA1 | 16 |
| mc21287 | ept-1 fxbA2 | This study |
| mc21289 | ept-1 fxbB4 | This study |
| mc21608 | ept-1 fxbB21::Tn10 | This study |
| mc21609 | ept-1 fxbC22::Tn10 | This study |
| mc21610 | ept-1 fxbB21::Tn10/pYUB563 | This study |
| mc21611 | ept-1 fxbC22::Tn10/pYUB563 | This study |
| Cosmids or plasmids | ||
| pKSII+ | Ampr; high-copy-number cloning vector, ColE1, unable to replicate in mycobacteria | Stratagene |
| pMD31 | Kanr; E. coli-mycobacterium shuttle vector | 11 |
| pYUB412 | Ampr Hygr; E. coli-mycobacterium shuttle vector; ColE1 ori; int attP nonreplicative but integration proficient in mycobacteria | 6 |
| pYUB415 | Ampr Hygr; E. coli-mycobacterium shuttle vector; ColE1 ori; M. fortuitum pAL5000 ori; lambda cos | 6 |
| pYUB340 | pYUB18 with a 30-kb insert which can complement exochelin mutant mc2848 | 16 |
| pYUB347 | pMD31 with a 4.3-kb fragment which can complement exochelin mutant mc2848 | 16 |
| pYUB563 | pYUB415 with a 31-kb insert from mc2155 in the BamHI site | This study |
| pYUB908 | pYUB563 fxbB::Tn10 | This study |
| pYUB909 | pYUB563 fxbC::Tn10 | This study |
| Phage λNK1316 | Mini-Tn10 kan/Ptac-ATS transposase | 20 |