TABLE 4.
Production of mycobactin grown under iron-deplete and -replete conditionsa
Strain | Relevant genotype | Mycobactin production (mg of mycobactin/g [wet wt] of cells)
|
Exochelin productionb | ||
---|---|---|---|---|---|
MM plus dipyridyl | MM | MM plus iron | |||
mc2155 | fxbA+fxbB+fxbC+ | 3.69 ± 0.57 | 1.63 ± 0.48 | 0.23 ± 0.21 | 1.11 ± 0.05 |
mc21608 | fxbB21::Tn10 | 7.12 ± 1.05 | 4.61 ± 0.86 | 0.32 ± 0.20 | 0.30 ± 0.10 |
mc21609 | fxbC22::Tn10 | 3.75 ± 0.68 | 2.53 ± 0.77 | 0.32 ± 0 | 0.18 ± 0.08 |
mc21610 | fxbB21::Tn10/pYUB563 | 3.44 ± 0.44 | 2.81 ± 0.37 | 0.30 ± 0.27 | 1.16 ± 0.11 |
mc21611 | fxbC22::Tn10/pYUB563 | 2.49 ± 0.07 | 1.68 ± 0.11 | 0.27 ± 0.32 | 1.13 ± 0.10 |
The bacteria were grown for 3 days in MM alone or MM supplemented with 50 μM 2,2′-dipyridyl (iron deplete) or with 50 μM iron (iron replete). The data are from a single experiment performed in triplicate and are representative of three separate experiments.
Exochelin production (measured by the change in optical density at 630 nm per milliliter) for cells grown in MM.