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. 2023 Nov 29;15(12):2344. doi: 10.3390/v15122344

Figure 2.

Figure 2

Recombinant expression of DWV His-Prot-Pol*. (A) Total E. coli protein of non-induced and induced cultures transformed with the plasmid pet11a-His-Prot-Pol* were resolved via SDS-PAGE and visualized via Coomassie stain. Note the overexpression of a protein with an apparent molecular mass of 60 kDa in the induced culture. (B) Western blot analysis of total E. coli protein of non-induced and induced cultures transformed with the plasmid pet11a-His-Prot-Pol*. Note the reactivity of the anti-His-tag Mab with the overexpressed protein. The recombinant protein is marked with an arrow (His-Prot-Pol*).