Table 1.

Oligonucleotides used in this study.

Name	Sequence
5′-3C-Prot_fwd	5′-GGATCAACACAACAAGTAGACGCTGCTGTG-3′
Internal-Pol_rev	5′-CTTAGACAACCTTGTTGCCAAATTTGTCCAC-3′
5′-3C-overhang-pet11a_rev	5′-GTCTACTTGTTGTGTTGATCCATGATGATGATGGTGAT-GGTG-3′
Internal-Pol-overhang-pet11a_fwd	5′-GCAACAAGGTTGTCTAAGTAGCTCGAGGATCCGGCTG-CTAAC-3′
NcoI-3′-3C_rev	5′-TTTCCATGGTTGAGCATGAGCTAGCTTTGCATCCACTCTACC 3′
NcoI-Stop-pet11a_fwd	5′-AAACCATGGTAGCTCGAGGATCCGGCTGCTAACA-3′
pet11a-overhang-5′-3C_fwd	5′-AGATATACATATGGGATCAACACAACAAGTAGACGCTG-3′
His-overhang-Internal-Pol _rev	5′-TGATGATGATGCTTAGACAACCTTGTTGCCAAATTTG-TC-3′
Internal-Pol-overhang-His_fwd	5′-TTGTCTAAGCATCATCATCATCATCATCACCACCAC-3′
5′-3C-overhang-pet11a_rev	5′-TGTGTTGATCCCATATGTATATCTCCTTCTTAAAGTTA-AAC-3′
3C-C2307A_fwd	5′-GATGGTGTTGCTGGTTCAATATTGTTG-3′
3C-C2307A_rev	5′-ACCAGCAACACCATCGCCGTGGTATGG-3′
Internal-Beta-Lactamase_rev	5′-GTCGTTTGGTATGGCTTCATTC-3′
Internal-Beta-lactamase_fwd	5′-GAATGAAGCCATACCAAACGAC-3′
3C-Q2393A_fwd	5′-CTCATGCTGCAAGCCCTTCTACTGG-3′
3C-Q2393A_rev	5′-TGCAGCATGAGCTAGCTTTGCATCCAC-3′
Flag-Internal-Hel_fwd	5′-GACTACAAAGACGATGACGACAAGGGATTGAAATAT-AGTGAAGCAGT-3′
His-3′-3C_rev	5′-GATGATGTTGAGCATGAGCTAGCTTTGCATCCAC-3′
ATG-Flag-pet11a_rev	5′-CTTGTCGTCATCGTCTTTGTAGTCCAT-3′
3C-overhang-His-tag_fwd	5′-TCATGCTCAACATCATCATCATCATCATCACCA-3′
3C-Q2118A_fwd	5′-ACTACTAAGCCTGCCGGATCAACACAACAAGTAGAC-3′
3C-Q2118A_rev	5′-GGCAGGCTTAGTAGTAACTGGCAATC-3′
3C-H2190A_fwd	5′-AAGTATATTGCTAATCAAGAGACTAGAATG-3′
3C-H2190A_rev	5′-CTCTTGATTAGCAATATACTTAAAATA-3′
3C-D2225A_fwd	5′-TCATTTGCGAGTAATATCGTGCTTGTGACT-3′
3C-D2225A_rev	5′-ATTACTCGCAAATGACTCCTCTCCCGC-3′
3C-H2170A_fwd	5′-TTGAGGGCATATATTGAGTCAACT-3′
3C-H2170A_rev	5′-AGTTGACTCAATATATGCCCTCAACATTAAACA-3′
3C-D2199A_fwd	5′-ATGTCTGGTGCGATTTCTGGTATTGAG-3′
3C-D2199A_rev	5′-CTCAATACCAGAAATCGCACCAGACAT-3′
3C-N2227A_fwd	5′-TCATTTGATAGTGCGATCGTGCTTGTGACT-3′
3C-N2227A_rev	5′-AGTCACAAGCACGATCGCACTATCAAATGA-3′