Table 1.
Selective species-specific primers for SIHUMI member strains and L. lactis MG1363.
| Target strain | Oligonucleotide Sequence (5′ to 3′) | Amplicon size | Reference |
|---|---|---|---|
| E. faecalis OG1RF | F: ACGGAGATTGTCACGCTTAGT R: TCGGCATTATCTGGGTGGTC |
122 bp | Buttimer et al. (2022) |
| E. coli LF82 | F: CGGGTGTTGTCCTAACTGCT R: CGAGTGGTCATTGGCCTCAT |
107 bp | Buttimer et al. (2022) |
| R. gnavus ATCC 29149 | F: GCGTGCTTGTATTCCGGATG R: GCCTGAACAGTTGCTTTCGG |
115 bp | Guerin et al. (2021) |
| F. prausnitzii A2-165 | F: TATTGCACAATGGGGGAAAC R: CAACAGGAGTTTACAATCCGAAG |
77 bp | Lengfelder et al. (2019) |
| P. vulgatus DSM1447 | F: AAGCAGCAGGGAAATGTGGA R: CTTTCCTTACTTGCGCGTCG |
142 bp | Buttimer et al. (2022) |
| L. plantarum WCFS1 | F: CGAAGAAGTGCATCGGAAAC R: TCACCGCTACACATGGAGTT |
71 bp | Lengfelder et al. (2019) |
| B. longum ATCC15707 | F: GAGGCGATGGTCTGGAAGTT R: CCACATCGCCGAGAAGATTC |
108 bp | Lawley et al. (2017) |
| L. lactis MG1363 | F: GCGATGAAGATTGGTGCTTGC R: ATCATCTTTGAGTGATGCAATTGC |
173 bp | This work |