Figure 1.

Initial AT evaluation strategy for identifying synaptic Abs validated in other applications. A, Common reference markers for presynaptic and postsynaptic locations and nuclei. B, Example evaluation of an Ab against GluA2 (Abcam ab206293), a glutamatergic receptor with a known postsynaptic localization at excitatory synapses. A single 70-nm section from adult mouse cortex, labeled with the GluA2 Ab (green) and synaptic markers PSD-95 (Cell Signaling 3450), synapsin (Synaptic Systems 106006), gephyrin (NeuroMab L106/93), GABA (Millipore AB175), and the nuclear label DAPI. The panel to the right is an enlarged view of the boxed area in the left panel. The GluA2 Ab was scored as excellent, based on its colocalization with PSD-95, adjacency to synapsin and minimal background label. See Extended Data Figures 1-1 and 1-2 for more details.