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. 2023 Dec 8;10(12):ENEURO.0290-23.2023. doi: 10.1523/ENEURO.0290-23.2023

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Copyright © 2023 Micheva et al.

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Figure 9. — The CBS assay has high predictive value for Ab success in AT experiments. A, Euler diagrams for projects L113 (Homer1), L109 (Calbindin), and L106 (Gephyrin). B, Table listing the percent of ELISA positive candidates giving rise to brain section AT positive mAbs (score ≥ 2.5) broken down by their performance on each validation assay. C, Ratio of the positive predictive value for AT suitable mAbs to the false omission rate shown for each validation assay. The CBS assay was most predictive for identifying brain AT positive mAbs. IF, immunofluorescence; IHC, immunohistochemistry; IB, immunoblot; AT, array tomography; CBS, cell-based proxy screen.