TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Sourcea | Relevant properties | Reference(s) |
|---|---|---|---|
| E. coli DH5α | GIBCO BRL | Host for E. coli plasmids | |
| E. coli plasmids | |||
| Bluescript pSK− | Stratagene | ||
| pHic | Cloned 468-bp fragment of R3001 purE-pepN region, obtained by PCR amplification with primers purE-F and pepN-R followed by subcloning of AluI partial digestion fragment into SmaI site of pSK− | This study | |
| pMCC1 | PstI (5.2-kb) fragment of the Hib Eagan hif operon in pUC19, subcloned from a cosmid library; includes the purE-hifA junction, hifA, hifB, and the 5′ end of hifC | This study; 13 | |
| H. influenzae strains | |||
| Eagan (E1A) | CSF from meningitis patient | Type b, streptomycin resistant, virulent in infant rat model | 38 |
| Rd (R906) = Goodgal | Nonencapsulated derivative of type d strain; avirulent in rat model | 8 | |
| INT1 | Blood from meningitis patient | Nonencapsulated; biotype V; virulent in rat model | 31 |
| R539 (ATCC 9006) | ATCC | Reference strain of serotype a | |
| R538 (ATCC 9795) | ATCC | Reference strain of serotype b | |
| R540 (ATCC 9007) | ATCC | Reference strain of serotype c | |
| R541 (ATCC 9008) | ATCC | Reference strain of serotype d | |
| R542 (ATCC 8142) | ATCC | Reference strain of serotype e | |
| R543 (ATCC 9796) | ATCC | Reference strain of serotype f | |
| U11 | CSF | Streptomycin-resistant derivative of U1 (Ramirez); nonencapsulated; avirulent in infant rat assay | 38 |
| R1965 (NCTC 8143) | NCTC | HK389; type strain of H. influenzae; nonencapsulated, biotype II | 22 |
| R1967 (ATCC 11116) | ATCC | Type strain of H. influenzae biotype aegyptius | |
| R2140 | Blood | H. influenzae biotype aegyptius isolate from BPF; hemagglutinating (CDC F3031) | 6 |
| R2141 | Blood | H. influenzae biotype aegyptius isolate from BPF; nonhemagglutinating (CDC F3035) | 6 |
| R2777 | CSF, from invasive disease | Nonencapsulated | This study |
| R3001 | Bronchial lavage of cystic fibrosis patient | Nonencapsulated | 25 |
| C2836 | Otitis media | Nonencapsulated | This study |
| C2840 | Tracheal infection in newborn | Nonencapsulated | This study |
| C2843 | Otitis media | Type b | This study |
| C2853 | Sputum from cystic fibrosis patient | Nonencapsulated | 25 |
| C2859 | CSF | Nonencapsulated | 25 |
| C2861 | CSF | Nonencapsulated | 25 |
a
ATCC, American Type Culture Collection; NCTC, National Collection of Type Cultures.