TABLE 3.
Junctions of C2861 deletions
| Deletiona | Deleted sequence (Hib AM30 nt) | Sequence at bordersb |
|---|---|---|
| Δ1 | 1477–4164 | ATTTTGAATTAAT…2687 bp…GTTTTGGATTAAA |
| AttttgGATTAAA | ||
| Δ2 | 4973–5102 | CACGCTAAAG…130 bp…AACGCCAAAT |
| CacgcCAAAT | ||
| Δ3 | 5230–5763 | GCCAGTGCCA…534 bp…CCCCGTGCCT |
| GccCGTGCCT | ||
| ΔhifE | 6327–7515 | GCTACCACA…1189 bp…CCTACAACT |
| GcgactacT |
a
Deletions are shown in Fig. 4.
b
For each deletion, the AM30 sequence is shown above the C2861 sequence. Matching Hib AM30 sequences at the right and left junctions are underlined, and the sequence within which an exchange is thought to have occurred is indicated in lowercase. The C2861 ΔhifE sequence differs from Hib AM30 junctions at two positions (the third and sixth from its 5′ end). Hib AM30 sequences are from reference 44 (accession no. Z33502). Hib AM30 coordinates are as follows: Δ1, nt 1474 to 1486 and 4162 to 4174; Δ2, nt 4968 to 4977 and 5098 to 5107; Δ3, nt 5228 to 5237 and 5762 to 5771; ΔhifE, nt 6324 to 6332 and 7510 to 7518.