Table 2.
The primer sequence of the gene.
| Gene | GenBank | Sequence (5′–3′) | TM °C |
|---|---|---|---|
| β-actin | NM_205518.2 | F: CATTGTCCACCGCAAATGCT R: AAGCCATGCCAATCTCGTCT |
57.2 |
| IL-6 | NM_204628.2 | F: CAGGACGAGATGTGCAAGAA R: TAGCACAGAGACTCGACGTT |
56.6 |
| IL-10 | NM_001004414.4 | F: CGCTGTCACCGCTTCTTCA R: CTTTGTTCTCATCCATCTTCTC |
57.0 |
| IL-1β | XM_046931582.1 | F: CGACATCAACCAGAAGTGCTT R: GTCCAGGCGGTAGAAGATGA |
56.8 |
| IL-22 | NM_001199614.1 | F: GCCCTACATCAGGAATCGCA R: TCTGAGAGCCTGGCCATTTC |
57.8 |
| IL-17A | NM_204460.2 | F: GAAGGTGATACGGCCAGGAC R: TGGGTTAGGCATCCAGCATC |
56.8 |
| IFN-β | NM_001024836.2 | F: TGCAACCATCTTCGTCACCA R: GGAGGTGGAGCCGTATTCT |
56.68 |
| IFN-γ | NM_205149.2 | F: ACACTGACAAGTCAAAGCCGC R: AGTCGTTCATCGGGAGCTTG |
58.6 |
| TNF-α | XM_046927265.1 | F: TGTGTATGTGCAGCAACCCGTAGT R: GGCATTGCAATTTGGACAGAAGT |
57.8 |
| MUC-2 | XM_040673077.2 | F: TTCATGATGCCTGCTCTTGTG | 58.0 |
| R: CCTGAGCCTTGGTACATTCTTGT | |||
| ZO-1 | XM_046925214.1 | F: CTTCAGGTGTTTCTCTTCCTCCTC | 56.6 |
| R: CTGTGGTTTCATGGCTGGATC | |||
| Claudin-1 | NM_001013611.2 | F: GGTATGGCAACAGAGTGGCT R: CAGCCAATGAAGAGGGCTGA |
57.0 |
| Occludin | XM_046904540.1 | F: GATGGACAGCATCAACGACC R: CATGCGCTTGATGTGGAAGA |
58.0 |
IL-6, interleukin-6; IL-10, interleukin-10; IL-1β, interleukin-1β; IL-22, interleukin-22; IL-17A, interleukin-17A; IFN-γ, interferon-γ; IFN-β, interferon-β; TNF-α, tumor necrosis factor-α; MUC-2, Mucin-2; ZO-1, Zonula occludens-1.