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Sanger sequencing of SPAST family 497. We performed RNA analysis from peripheral blood of Fam ID 497: II:1 and II:2. The line 1 show the amplification from the exon 10 to exon 13 in II:1 with two amplicon of 288 bp and 208 bp corresponding at wilde-type allele and loss of exon 12, line 2 and 3 show the amplification of II:2 and wild-type sample (288 bp). The 4 line shows negative control of PCR amplification.
