Table 4.

Overview of RNA-sequencing data and quality filtering

Sample	Raw reads	Valid reads	Valid bases (G)	Valid ratio (reads)	Q20 (%)	Q30 (%)
MSTN_1	56,366,592	51,526,252	7.73	91.41	98.16	95.09
MSTN_2	53,536,100	48,951,576	7.34	91.43	98.28	95.32
MSTN_3	48,419,310	44,191,280	6.63	91.26	98.19	95.15
MSTN_4	56,300,514	51,669,906	7.75	91.77	98.03	94.84
NC_1	50,135,852	46,455,136	6.97	92.65	98.30	95.37
NC_2	57,182,986	52,565,182	7.88	91.92	98.28	95.35
NC_3	56,482,390	51,992,736	7.80	92.05	98.30	95.43
NC_4	57,457,366	51,315,170	7.70	89.3	97.44	93.54