Fig. 4.

BAPTA-AM and ORM-10962 prevent the enhanced cell permeability in HCAECs undergoing 10 % stretch. Cells were pre-incubated for 2 h with vehicle, 100 nM ORM-10962, or 5 μM BAPTA-AM, before exposure to 5 % stretch or 10 % stretch. After 24 h, cell permeability assay and VE-cadherin staining were performed and the experiment flow chart is shown in Fig. 4A. Cell permeability was quantified using mean fluorescence intensity (MFI) and all the data were normalized to 5+Veh group (B, n = 6; C, n = 5). Representative images for VE-cadherin staining are shown in Fig. 4D. Data are presented as mean ± SD. P < 0.05, referred as *; ** for P < 0.01, *** for P < 0.001 and **** for P < 0.0001, were considered statistically significant.