Skip to main content
. 2023 Dec 1;69:102977. doi: 10.1016/j.redox.2023.102977

Fig. 2.

Fig. 2

In vitro characterization of cells with an inactivating mutation in Cys65 in Ref-1 in pancreatic cancer cells. mRNA (A) and protein levels (B) in Ref-1C65A expressing clone cells (E19, H7, J5, E14) with Cas9 Ref-1WT was used as a control line. One-way ANOVA, *p < 0.05, ***p < 0.001, Mean ± SEM, N = 3. C) The cell growth rate of Ref-1C65A expressing cells up to 96h, N = 3. Representative images of formation of colony in Ref-1C65A expressing cells (D), and quantification of number of colonies or Colony Formation Area (CFA) (E), One-way ANOVA, **p < 0.01, ***p < 0.001, Mean ± SEM, N = 3. F) Endonuclease activity of Ref-1 was assessed in Ref-1C65A expressing cells, N = 3. G) Cytotoxic effects of MMS were assessed in Ref-1C65A expressing cells, N = 3.