Fig. 6.

Mutation of Cys65 in Ref-1 dramatically changed the gene expression profile of PDAC cells and including downregulation of oxidative phosphorylation and upregulation of apoptosis genes as indicated by RNA sequencing. DEGs (FDR <0.05 & |logFC| > 1) identified in C65A clones, (A) E19 or (B) H7 compared with Cas9. Significant overlap of (C) up-regulated DEGs or (D) down-regulated DEGs in E19 and H7 compared with Cas9. (E) Selected Gene Ontology Biological Processes (GOBPs) based on Gene Set Enrichment Analysis (GSEA) notably over-represented in upregulated genes (NES >0) in E19 (left panel) or H7 (right panel) compared with Cas9, where the bar shows NES score of each GOBP), whereas the dot on the line indicates corresponding FDR-adjusted p value in the scale of -log10. The vertical dash lines represent FDR = 0.25. (F) GSEA) plots of selected hallmark gene sets or pathway or GOBP for E19 (left panels) or H7 (right panels) compared with Cas9.