figs3.
WTAP-mediated m6A modification contributed to HCC ferroptosis. WTAP siRNA and plasmid were utilized to transfect Huh7 and HepG2 cells respectively followed by 10 μM erastin or sorafenib. A-B Overall cellular proliferation tested by MTT assay. C Huh7 and HepG2 cells transfected with WTAP plasmid were treated with erastin with or without cell death inhibitors (Ferrostatin-1, 1 μM; Liproxstatin-1, 0.1 μM; Necrostatin-1, 10 μM; ZVAD-FMK, 10 μM; Necrosulfonamide, 0.5 μM) for 24 h. MTT assay was used to test overall cellular proliferation. The mean±SD was used as the value of three independent experiments. *p<0.05; **p<0.01; ***p<0.001.