figs4.

Autophagy activation mediated by m⁶A modification contributes to ferroptosis in HCC. A-B The fold change of ferrptosis- and autophagy-relate gene from RNA-Seq. C-D HepG2 and Huh7 were treated with erastin for 24 h after transfection with WATP siRNA and plasmid. Immunofluorescence detection of endogenous LC3 expression. E-G Expression of autophagy-related genes was detected by western blot. H MeRIP qPCR was used to detected the levels of m⁶A modification on autophagy-related genes. The mean±SD was used as the value of three independent experiments. *p<0.05; **p<0.01; ***p<0.001.