figs6.
ATG5 is a downstream target gene of WTAP-mediated m6A modification in HCC ferroptosis. Erastin was applied to HuH7 or HepG2 cells that had been transfected with WTAP siRNA and ATG5 plasmid alone or together. A Expression of autophagy-related genes was detected by western blot. B-C The mRNA expression of MAP1LC3B and SQSTM1 was detected by RT-PCR. D Immunofluorescence detection of endogenous LC3 expression in Huh7. E-H The level of iron, MDA, ROS, and GSH in HCC were tested detected by kits. The mean±SD was used as the value of three or four independent experiments. *p<0.05; **p<0.01; ***p<0.001.