figs8.

Expression of ATG5 in HCC ferroptosis. Huh7 and HepG2 cells treated with erastin, sorafenib and RSL3. A RT-PCR was used to detected ATG5 mRNA expression in Huh7. B Expression of ATG5 protein detected by western blot. C RT-PCR was used to detected ATG5 mRNA expression in HepG2. D Translation efficiency of ATG5 in HepG2, calculated as: translation efficiency = ATG5 protein level / ATG5 mRNA expression level. E Huh7 cells transfected with control or YTHDC2 plasmid were treated with CHX (100 μg/ml) and erastin (10 μM) for 24 h. The expression of ATG5 protein was detected at 0, 2, 4, 6 and 8 h respectively. F Schematic generation strategy for the Pmir-GLO luciferase reporters containing ATG5 CDS fragment. G Schematic view of the defined domains of YTHDC2 and YTH deletion truncations used. The mean±SD was used as the value of three independent experiments. *p<0.05; **p<0.01; ***p<0.001.