Fig. 1.

Levels of m⁶A modification is elevated in HCC ferroptosis. Ferroptosis inducers (10 μM Erastin, 10 μM Sorafenib, and 2.5 μM RSL3) were applied to Huh7 and HepG2 cells for 24 h with or without the addition of Ferrostatin-1 (1 μM), Necrostatin-1 (10 μM), and ZVAD-FMK (10 μM). A-B Transwell assays were used to assess the capacity for invasion. C Wound healing assay was performed to detect Huh7 cell migration. D Intracellular proliferation was detected using the Edu kit. E Overall cellular proliferation tested by MTT assay. F–H The level of MDA, iron, and GSH were tested detected by kits. I m⁶A modification level was detected by dot blot. J m⁶A modification level was detected by m⁶A RNA Methylation Quantitative kit. The mean ± SD was used as the value of three or four independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.