Fig. 2.

WTAP-mediated m⁶A modification contributed to HCC ferroptosis. Ferroptosis inducers (10 μM Erastin, 10 μM Sorafenib, and 2.5 μM RSL3) were applied to Huh7 and HepG2 cells for 24 h. A-B Expression of m⁶A writers and erasers in ferroptoic HCC cells detected by Western blot. C WTAP siRNA and plasmid were utilized to transfect Huh7 and HepG2 cells respectively followed by 10 μM erastin. m⁶A RNA Methylation Quantitative kit was utilized to detect m⁶A modification level. D Transmission electron microscopic observation of mitochondrial morphology in Huh7. Scale bars: 500 nm. E-H The level of MDA, iron, and GSH were tested detected by kits. The mean ± SD was used as the value of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.