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. 2023 Dec 1;69:102971. doi: 10.1016/j.redox.2023.102971

Fig. 6.

Fig. 6

Erastin promotes HCC PDO ferroptosis in an m6A-dependent manner. A Representative bright-field images of HCC PDOs from three individuals that were exposed to erastin at the indicated concentration at 0, 3, 5 days are shown. Scale bar, 0.1 mm. B m6A modification level in PDOs was detected by dot blot. C MeRIP-PCR detected m6A level on ATG5 mRNA. D-F The mRNA expression of WTAP, YTHDC2, PTGS2 was detected by RT-PCR. G-I The level of GSH, MDA and iron in PDOs was detected by kits. J-L The mRNA expression of FTH, MAP1LC3, SQSTM1 was detected by RT-PCR. M The protein expression of ATG5 in PDOs was detected by Western blot. N The mRNA expression of ATG5 was detected by RT-PCR. O Translation efficiency of ATG5 in PDOs. Calculated as: translation efficiency = ATG5 protein level/ATG5 mRNA expression level. The mean ± SD was used as the value of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.