FIG. 1.

The bgl operon showing all three genes required for regulated uptake and utilization of β-glucosides. The identified promoters are designated P0, P1, and P2, and the rho-independent transcription terminators are designated T1 and T2. Two primers, FP2 and RP2, were used to identify bglG mRNA by RT-PCR. In plasmid pIX41 the bgl fragment that contains the 5′ end of bglG, promoter P2, terminator t2, and the 3′ end of bglF is cloned upstream of the promoterless cat gene. Primers designated FP1 and RP1 were used for RT-PCR identification of the cat gene. A reverse primer, SP1, was used for sequencing. ORF, open reading frame.