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RT-PCR amplification of bglG mRNA. Lane 1, PCR-amplified bglG, using total DNA from strain i484; lane 2, RT-PCR with RNA isolated from uninfected mouse liver; lane 3, RT-PCR with RNA isolated from in vitro-grown strain i673; lane 4, PCR amplification of RNase-treated total RNA from infected mouse liver; lane 5, RT-PCR with total RNA from infected mouse liver; lane M, molecular weight marker.
