Table 2.

Steps within the PCR program.

Initialization	94°C, 2 min
	5 cycles	5 cycles	5 cycles	5 cycles
Denaturation	94°C, 30 s	94°C, 30 s	94°C, 30 s	94°C, 30 s
Annealing	62°C, 30 s	61°C, 30 s	59°C, 30 s	58°C, 30 s
Elongation	72°C, 1 min	72°C, 1 min	72°C, 1 min	72°C, 1 min