FIGURE 2.

In vitro transcription reactions with a UTP feed resulted in production of mRNA with the lowest level of dsRNA. (A) J2 dotblot of in vitro transcription reactions with a dinucleotide cap analog and a DNA template encoding a poly(A)-tail at the 3′ end, in which different nucleotide triphosphates as indicated were fed over time: (B) J2 dotblot of dsRNA levels from in vitro transcription RNA from DNA templates encoding different 3′ ends either with no nucleotide triphosphate feed or with a UTP feed: Quantification of dsRNA levels as shown in (C, D). Representative example of several experiments with similar outcomes.