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. 2023 Dec 7;14:1293424. doi: 10.3389/fpls.2023.1293424

Figure 2.

Figure 2

Establishment of leaf polarity. The developing leaf primordium has three domains, and the transcription factors in the three domains inhibit each other’s expression and control each other. Transcripts of AS1/AS2, HD-ZIPIII and tasi-ARF accumulate in the adaxial domain of the leaf primordium, transcripts of ARF 3/ARF4, KAN and miR165/166 accumulate in the abaxia domain, and WOX1/PRS are expressed in the intermediate domain of the leaf primordium. AGO10 inhibits miR165/166, AGO1 regulates miR165/166 and miR165/166 inhibits HD-ZIPIII. AGO7 stabilizes ta-siR-ARF, and ta-siR-ARF degrades of ARF2/3/4. tasi-ARF and miR165/166 Rnas can move between cells and inhibit ARF2/3/4 and HD-ZIPIII after transcription, and ARF2/3/4 is controlled by auxin. KAN and HD-ZIPIII antagonized each other, and ARF2/3/4 and KAN were inhibited by AS1/AS2. KAN inhibited the expression of WOX1 and PRS, while WOX1 and PRS inhibited the expression of KAN. Adaxial expression of MP and off-axes enrichment of auxin together localized WOX1 and PRS expression in the intermediate domain. In addition, MP may be the direct target of positively expressed HD-ZIPIII, and YAB promotes the expression of WOX1/PRS with KAN and ARF2/3/4, while YAB promotes the expression of WOX1/PRS (Barkoulas et al., 2007; Kalve et al., 2014; Du et al., 2018; Wang et al., 2021a).