Table 2.
Antibodies used in ADCC assay and flow cytometry.
| Target | Fluorochrome | Clone | Dilution | Vendor | RRID |
|---|---|---|---|---|---|
| IFNγ | PE | B27 | 1:150 | BD Biosciences | AB_395518 |
| CD3 | FITC | UCHT1 | 1:200 | BioLegend | AB_314060 |
| CD3 | BUV395 | SK7 | 1:200 | BD Biosciences | AB_2744382 |
| CD4 | APC-H7 | RPA-T4 | 1:200 | BD Biosciences | AB_1645478 |
| CD4 | PerCP/Cyanine5.5 | RPA-T4 | 1:200 | Biolegend | AB_893328 |
| CD8 | PerCP/Cyanine5.5 | RPA-T8 | 1:200 | BD Biosciences | AB_1727513 |
| CD8 | FITC | HIT8a | 1:200 | BD Biosciences | AB_395996 |
| CD45RA | Alexa Fluor 700 | HI100 | 1:150 | BD Biosciences | AB_1727496 |
| CCR7 | APC | G043H7 | 1:150 | Biolegend | AB_10917387 |
| TNFα | PE-Cy7 | MAb11 | 1:150 | BD Biosciences | AB_396764 |
| IFNγ | APC | B27 | 1:150 | BD Biosciences | AB_398580 |
| IFNγ | BV786 | 4S.B3 | 1:150 | Biolegend | AB_11219192 |
| CD107a | APC | H4A3 | 1:150 | Biolegend | AB_1279057 |