Fig. 2.

Bacteria were observed in the dermis of keloid. (a) HE and Masson's trichrome representative staining of keloid tissue and normal skin tissue, 100×. Scale bar = 100 μm. (b) Fluorescence in situ hybridization experiments were performed on the dermis and fat layer of skin. Keloid: keloid tissues. Control: normal tissues. 100×. Scale bar = 50 μm. (c) Quantitative statistics of EUB338 expression via FISH experiments. Unpaired t test was used. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. Keloid (n = 4), Control (n = 5). (d) Electron microscopic view of bacteria in the dermis of keloids, red arrows. Scale bar = 0.5 μm.