Table 3.
Data processing statistical results.
Group name | Qualified | Nochime | AvgLen | Q20 | Q30 | GC% | Effective% |
---|---|---|---|---|---|---|---|
Blank | 76096 | 71639 | 421 | 97.91 | 93.34 | 51.36 | 88.96 |
Model | 77179 | 72435 | 420 | 98.17 | 94.00 | 51.34 | 91.50 |
LLP | 75029 | 69677 | 425 | 97.87 | 93.19 | 50.63 | 88.69 |
PCP | 77432 | 69355 | 412 | 98.33 | 94.43 | 51.88 | 87.28 |
EP | 79477 | 74748 | 423 | 97.68 | 92.72 | 50.14 | 91.21 |
AMX | 77150 | 74212 | 405 | 98.26 | 94.10 | 52.93 | 90.69 |
Qualified is the sequence after Raw Tags filter low quality and short length; Nochime is the Tags sequence that is finally used for subsequent analysis after filtering chimeras, namely Effective Tags. The base is the number of bases in the final Effective Tags. AvgLen is the average length of Effective Tags; Q20 and Q30 are the percentages of bases in Effective Tags with base quality values greater than 20 (sequencing error rate less than 1%) and 30 (sequencing error rate less than 0.1%). GC (%) represents the content of GC bases in Effective Tags. Effective (%) represents the percentage of the number of Effective Tags to the number of Raw PE.