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Integration of plasmid pDS226b into the chromosome of strain DS988. (A) Schematic representation of the pDS226b integration into the DS988 chromosome. (B) Southern blot hybridization of total genomic DNA from UW136 (lane 1), DS988 (lane 2), and DS988::pDS226b (lane 3) digested with ClaI and HindIII with ptsP as probe. The hybridizing fragments in panel B are as indicated in panel A.
