TABLE 1.
Effect of ptsP mutation on different enzymatic activitiesa
| Strain | Activity
|
|||||
|---|---|---|---|---|---|---|
| Glucose uptake (nmol min−1 mg−1) | Glucose kinase (nmol min−1 mg−1) | Glucose-6-phosphate dehydrogenase (nmol min−1 mg−1) | Nitrogenase on glucose (nmol h−1 g−1) | Nitrogenase on gluconate (nmol h−1 g−1) | β-Ketothiolase (nmol h−1 g−1)b | |
| UW136 | 4.01 ± 0.56 | 19.7 ± 2 | 311.2 ± 10.7 | 1,096 ± 50 | 9,097 ± 260 | 2,980 ± 138 |
| DS988 | 5.02 ± 0.31 | 18.8 ± 3.2 | 256.1 ± 48.0 | NDc | 4,200 ± 612 | 1,223 ± 36 |
a
Cells were grown for 20 h on BS, washed with the same medium without a carbon source, and incubated for 4 h on Burk’s medium supplemented with 11 mM glucose or gluconate. Values are the means of three determinations.
b
β-Ketothiolase activity was determined from cells grown for 48 h in liquid PY-sucrose medium.
c
ND, not detected.