Figure 5.

LSFM image-based data processing flow for analyzing the spatial correlations in the lymphoma TMEs. (A) Post analysis pipeline of the multiplexed LSFM images. (B) The density (cells per mm³) of immune cell types in lymph nodes from three donors with RLN, DLBCL and AITL. i) B cells and T cells: CD20, blue; CD8, red. ii) Tregs and T cells: FOXP3, green; CD8, red. iii) Macrophages, TAMs and T cells: CD68, blue; CD163, red; CD3, green. iv) Monocytes, neutrophils and T cells: CD14, blue; CD15, red; CD3, green. (C) The number of T cells around each immune cell was plotted as a function of the radial distance from the immune cell. Different color bars represent different diseases with blue for RLN, green for DLBCL, and red for AITL.