Figure 1.

Protocol design across experimental days. Ninety animals were submitted to the ethanol two-bottle choice procedure acquisition phase, during which they were exposed to individual cages with access to two bottles, one of water and one of 10% ethanol solution, for 15h, every other day. At the end of the acquisition phase, the 42 animals that showed the highest ethanol drinking/preference were randomly assigned to 4 groups: Veh-Veh, M100-Veh, Veh-Aya or M100-Aya. Animals were then submitted to the treatment phase, with each treatment phase being followed by 3 re-exposure tests.