FIG. 1.

Schematic drawing showing the genetic organization, restriction map, and protein-encoding regions of the chromosomal site affected in Synechocystis mutant 11 (A), the corresponding site of the WT of Synechocystis (C) (17), and the insertion sites of the aphII and smr genes in selected sites of the different genes to obtain directed mutants of different ORFs (B). Shaded arrows represent genes encoding putative proteins in Synechocystis; empty arrows represent genes encoding hypothetical proteins in Synechocystis; small arrows labeled with aphII represent inserted aphII gene cassettes; small arrows labeled with smr represent inserted smr gene cassettes; shaded boxes represent regions sequenced in this work and sequences L77077, M11Kan5, and M11Kan3; asterisks indicate partial deleted genes; a long horizontal black arrow represents the deletion that occurred during integration of an aphII gene in mutant 11. Bl, BlpI; Bs, BstEII.