FIG. 2.

PCR analyses using chromosomal DNA from the WT (lanes 1, 4, 6, 8, and 10) and mutants (ΔGK2 [lane 2], ΔGS2 [lane 3], DK2 [lane 5], ΔKK2 [lane 7], and DS2ΔKK2 [lanes 9 and 11]) (see Table 1) of Synechocystis as a template and primers specific for selected genes ggpS5 and ggpS3 [lanes 1 to 3], glpD5 and glpD3 [lanes 4, 5, 10, and 11], and glpK5 and glpK3 [lanes 6 to 9]) in order to verify the lesions in the chromosomal DNA of the mutants. Lane M, fragment size marker, EcoRI/HindIII-cut λ DNA.