FIG. 5.

Overexpression of GGPS (Sll1566) from Synechocystis in E. coli. (A) Coomassie blue-stained gel from electrophoretic separation of soluble proteins from different fractions of the purification procedure; (B and C) TLC separation of reaction products obtained after determination of GGPS activity by using soluble proteins from different fractions of the purification procedure. The reaction products were directly applied to the TLC plate (B) or pretreated with alkaline phosphatase (C). Lanes: 1, proteins extracted from E. coli harboring the empty pBAD/HisB vector; 2, proteins extracted from E. coli cells harboring the ggpS gene on the pBADGGPS vector; 3, purified GGPS protein with the His tag; 4, purified GGPS protein that was dialyzed overnight against low-salt-containing buffer; M, marker lanes (protein broad-range marker from Bio-Rad [A] and ¹⁴C-labeled GG isolated from salt-stressed Synechocystis cells [B and C]).