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. 2023 Dec 22;14:1009–1033. doi: 10.18632/oncotarget.28546

Figure 6. Long-range haplotyping of the Xq27 region in prostate cancer patients and healthy individuals.

Figure 6

(A) TAR isolation of the SPANXC gene. The positions of SPANX genes are shown. The genes (in black) share 98% identity and reside within large segmental duplications (SDs) (in orange) with >95% identity. The SPANXC gene was TAR-isolated as an 83 kb segment from 21 patients and 40 healthy individuals in one TAR cloning experiment. The targeting hooks were chosen from the sequences outside the SD. The same strategy was used in mutational analysis of other gene family members. Orientation of the genes in the SDs towards the centromere (cen) and telomere (tel) is indicated: SPANXD to SPANXC and SPANXA1 to SPANXC. (B) Gene conversion events between the SPANX-A/D gene family members (indicated by black arrows). (C) Six (I–VI) SPANX long-range haplotypes identified in the patients. Each column represents one of SPANX genes. For example, analysis of TAR isolates from individual VI revealed 10 copies of SPANXB at the SPANXB locus and conversion of SPANXD to SPANXC at the SPANXC locus.