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| Capture antigen (stock concentration, µg/mL) | Working concentration (µg/mL) | Volume of antigen (µL) | DPBS (µL) | Total (µL) |
|---|---|---|---|---|
| Spike/Nucleoprotein/RBD (500 µg/mL) | 3 | 36 | 5,964 | 6,000 |
Note: This dilution is for one plate. Multiply volumes by the number of plates in the assay.
