Table 3. IgG standard serial dilution in assay buffer.

. Adjust the volumes depending on the plate plan and the number of plates.Initial 1:100 dilution of human IgG

Prepare a 1:100 dilution of human IgG in the appropriate buffer prior to preparing the serial dilutions
	Dilution	Stock (mg/mL)	Stock (µL)	Assay buffer (µL)		Total (µL)
Human IgG	1:100	4.52	2	198		200
Serial dilution of human IgG following the initial 1:100 dilution
Concentration (ng/mL)		IgG Std (µL)	Assay buffer (µL)	Total (µL)	Take over (µL)	Left over (µL)
Std curve human IgG (10-fold dilution)	1,000	20	884	904	90	814
	100	90	810	900	90	810
	10	90	810	900	90	810
	1	90	810	900	90	810
	0.1	90	810	900	90	810
	0.01	90	810	900	90	810
	0.001	90	810	900	-	900