Table 4. IgA standard serial dilution in assay buffer.

Adjust the volumes depending on the plate plan and the number of plates.Initial 1:100 dilution of human IgA

Prepare a 1:100 dilution of human IgA in the appropriate buffer prior to preparing the serial dilutions
	Dilution	Stock (mg/mL)	Stock (µL)	Assay buffer (µL)		Total (µL)
Human IgA	1:100	2.0	2	198		200
Serial dilution of human IgA following the initial 1:100 dilution
Concentration(ng/mL)		IgA Std (µL)	Assay buffer (µL)	Total (µL)	Take over (µL)	Left over (µL)
Std curve human IgA (10-fold dilution)	1,000	45	855	900	90	810
	100	90	810	900	90	810
	10	90	810	900	90	810
	1	90	810	900	90	810
	0.1	90	810	900	90	810
	0.01	90	810	900	90	810
	0.001	90	810	900	-	900